去细胞化
核心
组织工程
壳聚糖
细胞生物学
细胞外基质
化学
自愈水凝胶
椎间盘
脚手架
基质(化学分析)
转化生长因子
生物医学工程
解剖
生物
生物化学
医学
有机化学
色谱法
作者
Wenzhong Kuang,Chen Liu,Hongguang Xu
出处
期刊:Cytotechnology
[Springer Nature]
日期:2021-04-15
卷期号:73 (3): 447-456
被引量:7
标识
DOI:10.1007/s10616-021-00469-0
摘要
Low back pain caused by intervertebral disc degeneration has become a global problem that seriously affects public health. The application of nucleus pulposus tissue engineering to disc degeneration has attracted increasing attention. A scaffold is important for nucleus pulposus tissue engineering, which provides a three-dimensional growth space with an appropriate biomechanical and biochemical microenvironment for seed cell differentiation and proliferation. In this study, a decellularized nucleus pulposus matrix/chitosan (DNPM/chitosan) hydrogel scaffold was prepared with crosslinker genipin. Nucleus pulposus stem cells (NPSCs) were cultured in hybrid hydrogels with or without transforming growth factor-β3 (TGF-β3) and then cell morphology, proliferation, and nucleus pulposus-related gene expression were analyzed. TGF-β3 was successfully incorporated into the DNPM/chitosan hydrogel and NPSCs grew well on both kinds of hydrogel. Moreover, gene expression of collagen-I, collagen-II, and aggrecan was enhanced in the DNPM/chitosan hydrogel with TGF-β3. These results indicate that the DNPM/chitosan hybrid hydrogel is a promising candidate scaffold for nucleus pulposus tissue engineering.
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