Single-cell and single-nucleus RNA-seq uncovers shared and distinct axes of variation in dorsal LGN neurons in mice, non-human primates, and humans

生物 神经科学 猕猴 电池类型 视皮层 灵长类动物 小细胞细胞 转录组 丘脑 核心 基因 细胞 基因表达 遗传学
作者
Trygve E. Bakken,Cindy Tj van Velthoven,Vilas Menon,Rebecca D. Hodge,Zizhen Yao,Thuc Nghi Nguyen,Lucas T. Graybuck,Gregory D. Horwitz,Darren Bertagnolli,Jeff Goldy,Anna Marie Yanny,Emma Garren,Sheana Parry,Tamara Casper,Soraya I. Shehata,Eliza Barkan,Aaron Szafer,Boaz P. Levi,Nick Dee,Kimberly A. Smith,Susan M. Sunkin,Amy Bernard,John W. Phillips,Michael Hawrylycz,Christof Koch,Gabe J. Murphy,Ed Lein,Hongkui Zeng,Bosiljka Tasic
出处
期刊:eLife [eLife Sciences Publications, Ltd.]
卷期号:10 被引量:43
标识
DOI:10.7554/elife.64875
摘要

Abundant evidence supports the presence of at least three distinct types of thalamocortical (TC) neurons in the primate dorsal lateral geniculate nucleus (dLGN) of the thalamus, the brain region that conveys visual information from the retina to the primary visual cortex (V1). Different types of TC neurons in mice, humans, and macaques have distinct morphologies, distinct connectivity patterns, and convey different aspects of visual information to the cortex. To investigate the molecular underpinnings of these cell types, and how these relate to differences in dLGN between human, macaque, and mice, we profiled gene expression in single nuclei and cells using RNA-sequencing. These efforts identified four distinct types of TC neurons in the primate dLGN: magnocellular (M) neurons, parvocellular (P) neurons, and two types of koniocellular (K) neurons. Despite extensively documented morphological and physiological differences between M and P neurons, we identified few genes with significant differential expression between transcriptomic cell types corresponding to these two neuronal populations. Likewise, the dominant feature of TC neurons of the adult mouse dLGN is high transcriptomic similarity, with an axis of heterogeneity that aligns with core vs. shell portions of mouse dLGN. Together, these data show that transcriptomic differences between principal cell types in the mature mammalian dLGN are subtle relative to the observed differences in morphology and cortical projection targets. Finally, alignment of transcriptome profiles across species highlights expanded diversity of GABAergic neurons in primate versus mouse dLGN and homologous types of TC neurons in primates that are distinct from TC neurons in mouse.
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