A Highly Sensitive and Selective Label‐free Electrochemical Biosensor with a Wide Range of Applications for Bisphenol A Detection

Abstract A label‐free DNA‐based electrochemical biosensor owning high sensitivity and selectivity has been established for detecting bisphenol A in a wide range of applications. Coupling the high electrochemical performance of graphene oxide‐thionine‐Au nanomaterial with the specific binding capacity of the aptamers to BPA, the monitoring of trace amount of BPA was realized, the detection limit was 3.3 pg ⋅ mL −1 with strong anti‐interference. Besides, using molecular docking, it was found that BPA binds to the bases DC‐49, DC‐51, DG‐52, DG‐53 and DA‐63 on the aptamer via hydrogen bonding and π‐π stacking interactions. Finally, the biosensor had been successfully applied in different real samples.