突变体
普通大麦
人口
化学
耕作
大麦
小麦族
转录因子
硝酸还原酶
硝酸盐
氮气
生物化学
生物
氮同化
细胞生物学
植物
非生物胁迫
基因
脱落酸
拟南芥
遗传学
禾本科
基因组
生态学
人口学
社会学
作者
Yangyang Gao,Shuxuan Quan,Bo Lyu,Tian Tian,Zhiguang Liu,Zhentian Nie,Shengdong Qi,Jingbo Jia,Jing Shu,Edwin P. Groot,Jiajie Wu,Yong Wang
摘要
Plants have evolved complex mechanisms to adapt to the changing nitrogen levels in the environment. In Arabidopsis, more than a dozen nitrate signaling regulatory genes have been characterized, including the NODULE INCEPTION-LIKE PROTEIN (AtNLP) genes, which play essential roles in nitrate signaling. However, whether NLP genes in the Triticeae crops are involved in nitrate regulation and nitrogen use efficiency (NUE) remains unknown. Here, we isolated a barley (Hordeum vulgare L.) mutant, hvnlp2-1, from a TILLING (Targeting Local Lesions IN Genomes) population and constructed two RNAi lines, hvnlp2-2 and hvnlp2-3, to study the function of HvNLP2. The expression of the nitrate-responsive genes was substantially inhibited after nitrate treatment in the hvnlp2 mutants, indicating that HvNLP2 controls nitrate signaling. Nitrate content was significantly higher in the hvnlp2 mutants, which may result from the decreased assimilation of nitrogen caused by reduced nitrate reductase activity and expression of nitrate assimilatory genes. HvNLP2 is localized to the nucleus in the presence of nitrate. Further investigation showed that HvNLP2 binds to and activates the nitrate-responsive cis-elements. Moreover, hvnlp2 exhibited reduced biomass, seed yield, and NUE. Therefore, HvNLP2 controls nitrate signaling and plays an important role in NUE.
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