清晨好,您是今天最早来到科研通的研友!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您科研之路漫漫前行!

Integrated gas chromatography‐mass spectrometry and ultra‐high‐performance liquid chromatography‐mass spectrometry renal metabolomics and lipidomics deciphered the metabolic regulation mechanism of Gushudan on kidney‐yang‐deficiency‐syndrome rats

代谢组学 脂类学 脂质体 嘌呤代谢 化学 代谢组 代谢途径 甘油磷脂 脂质代谢 生物化学 新陈代谢 内分泌学 色谱法 生物 磷脂
作者
Qing Lu,Jing Zhang,Ling Xin,Yanwei Lou,Feng Qin,Longshan Zhao,Zhili Xiong
出处
期刊:Journal of Separation Science [Wiley]
卷期号:46 (13) 被引量:10
标识
DOI:10.1002/jssc.202300124
摘要

Kidney-yang-deficiency-syndrome is a neuroendocrine disease caused by the dysfunction of the adrenal-pituitary-target gland axis. Gushudan is a traditional Chinese medicine prescription with the functions of tonifying the kidney and strengthening bone, and its bone-strengthening effect has been confirmed by previous anti-osteoporosis research. However, its kidney-tonifying mechanism has not been clear so far. In this study, renal metabolomics and lipidomics based on gas chromatography-mass spectrometry and ultra-high-performance liquid chromatography-high resolution mass spectrometry were integrated to find the metabolic disorders in kidney-yang-deficiency-syndrome rats. Protein precipitation and liquid-liquid extraction were used to extract metabolome and lipidome from the kidney. Gushudan regulated abnormal levels of amino acids, lipids, purines, and carbohydrates, such as L-arginine, hypoxanine, stearic acid, and phosphatidylethanolamine (P-18:1/20:4), which had effects on many metabolic pathways, such as glycerophospholipid metabolism, sphingolipid metabolism, glycine, serine and threonine metabolism and purine metabolism, and so forth. By integrating metabolomics and lipidomics, this study comprehensively revealed the abnormal metabolic activities of amino acids, lipids, and nucleotides in kidney-yang-deficiency-syndrome, and the metabolic regulation mechanism of Gushudan in preventing kidney-yang-deficiency-syndrome, as well as the improvement of Gushudan in maintaining renal cell structure, mitochondrial function, and energy supply, which also provided some new evidence and connotation for "kidney-bone" axis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
蔡从安完成签到,获得积分20
10秒前
傲娇而又骄傲完成签到 ,获得积分10
19秒前
精明晓刚发布了新的文献求助10
25秒前
星辰大海应助精明晓刚采纳,获得10
38秒前
Joeswith完成签到,获得积分10
52秒前
优美的明辉完成签到 ,获得积分10
58秒前
帅气的沧海完成签到 ,获得积分10
1分钟前
jlwang完成签到,获得积分10
1分钟前
1分钟前
彦嘉发布了新的文献求助10
1分钟前
宇文雨文完成签到 ,获得积分10
1分钟前
2分钟前
末末完成签到 ,获得积分10
2分钟前
2分钟前
ceeray23发布了新的文献求助20
2分钟前
科研通AI2S应助科研通管家采纳,获得10
2分钟前
笨笨完成签到 ,获得积分10
2分钟前
芒芒发paper完成签到 ,获得积分10
3分钟前
顺心蜜粉发布了新的文献求助30
3分钟前
顺心蜜粉完成签到,获得积分10
3分钟前
3分钟前
CC发布了新的文献求助10
3分钟前
淞淞于我完成签到 ,获得积分10
3分钟前
Jenny发布了新的文献求助50
3分钟前
CC完成签到,获得积分10
3分钟前
天天开心完成签到 ,获得积分10
3分钟前
Qian完成签到 ,获得积分10
4分钟前
4分钟前
玄之又玄完成签到,获得积分10
5分钟前
糯米团的完成签到 ,获得积分10
5分钟前
爆米花应助ceeray23采纳,获得20
5分钟前
爆米花应助彦嘉采纳,获得10
5分钟前
6分钟前
ceeray23发布了新的文献求助20
6分钟前
zpc猪猪完成签到,获得积分10
6分钟前
Jenny发布了新的文献求助50
6分钟前
香蕉觅云应助ceeray23采纳,获得20
6分钟前
hoshi完成签到 ,获得积分10
6分钟前
woxinyouyou完成签到,获得积分0
6分钟前
思源应助方俊驰采纳,获得10
6分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990568
求助须知:如何正确求助?哪些是违规求助? 3532220
关于积分的说明 11256532
捐赠科研通 3271057
什么是DOI,文献DOI怎么找? 1805207
邀请新用户注册赠送积分活动 882302
科研通“疑难数据库(出版商)”最低求助积分说明 809234