Isolation and Differentiation of Adipocyte Precursors Derived from Neonatal Murine Brown Adipose Tissue

Brown adipose tissue (BAT) is an important regulator of energy homeostasis. Primary brown adipocyte culture provides a powerful and physiologically relevant tool for in vitro studies related to BAT. Here, we describe a detailed procedure for isolation and differentiation of adipocyte precursors from neonatal murine interscapular BAT (iBAT).