Actomonas aquatica gen. nov., sp. nov., a marine lineage in the phylum Verrucomicrobiota with genetic potential for carbon and nitrogen fixation

A novel bacterial strain, designated WL0086 T , was isolated from a marine sediment sample collected in Lianyungang city, Jiangsu province, PR China. This strain showed the highest 16S rRNA gene sequence similarity to Geminisphaera colitermitum TAV2 T (92.7 %) of the family Opitutaceae , and all the unclassified cultured and uncultured isolates with similarities >95 % were from marine environments. Cells were Gram-stain-negative, aerobic, non-motile cocci with a size of 0.6–0.8 µm in diameter. Strain WL0086 T was positive for both oxidase and catalase, and grew at 20–37 °C (optimum, 28 °C), with 1.5–11.0 % NaCl (w/v; optimum, 2.5–4.0 %) and at pH 5.0–9.0 (optimum, pH 7.0). The major polar lipid profile of strain WL0086 T consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylcholine. The major isoprenoid quinone was menaquinone-7 and the predominant fatty acids were iso-C 14 : 0 , anteiso-C 15 : 0 , C 16 : 0 and C 16 : 1 ω 9 c . The complete genome consisted of a chromosome with 6 109 182 bp. The G+C content of genomic DNA was 64.0%. Results of phylogenomic analysis based on the 16S rRNA gene sequence and the whole genome suggested that strain WL0086 T formed a distinct clade closely neighbouring the members of the family Opitutaceae . On the basis of phylogenetic, phenotypic, and chemotaxonomic evidences, strain WL0086 T should represent a novel genus of the family Opitutaceae , for which the name Actomonas aquatica gen. nov., sp. nov. is proposed. The type strain is WL0086 T (=MCCC 1K05844 T =JCM 34677 T =GDMCC 1.2411 T ).