注释
计算机科学
人工智能
深度学习
排名(信息检索)
基因
拟南芥
基因亚型
光学(聚焦)
秩(图论)
计算生物学
机器学习
数据挖掘
生物
遗传学
数学
物理
组合数学
光学
突变体
作者
Sitao Zhu,Yuan Shu,Ruixia Niu,Yulu Zhou,Zhao Wang,Guoyong Xu
标识
DOI:10.1016/j.jgg.2024.03.004
摘要
A 5′-leader, known initially as the 5′-untranslated region, contains multiple isoforms due to alternative splicings (aS) and transcription start sites (aTSS). Therefore, a representative 5′-leader is demanded to examine the embedded RNA regulatory elements in controlling translation efficiency. Here, we develop a ranking algorithm and a deep-learning model to annotate representative 5′-leaders for five plant species. We rank the intra- and inter-sample frequency of aS-mediated transcript isoforms using the Kruskal-Wallis test-based algorithm and identify the representative aS-5′-leader. To further assign a representative 5′-end, we train the deep-learning model 5′leaderP to learn aTSS-mediated 5′-end distribution patterns from cap-analysis gene expression (CAGE) data. The model accurately predicts the 5′-end, confirmed experimentally in Arabidopsis and rice. The representative 5′-leader-contained gene models and 5′leaderP can be accessed at RNAirport (http://www.rnairport.com/leader5P/). This stage 1 5′-leader annotation records 5′-leader diversity and will pave the way to Ribo-Seq ORF annotation, identical to the project recently initiated by human GENCODE.
科研通智能强力驱动
Strongly Powered by AbleSci AI