Combining Reflectance Confocal Microscopy with Optical Coherence Tomography for Noninvasive Diagnosis of Skin Cancers via Image Acquisition

基底细胞癌 光学相干层析成像 医学 皮肤癌 病理 活检 黑色素瘤 放射科 基底细胞 癌症 癌症研究 内科学
作者
Ucalene Harris,Milind Rajadhyaksha,Manu Jain
出处
期刊:Journal of Visualized Experiments [MyJoVE Corporation]
卷期号: (186) 被引量:3
标识
DOI:10.3791/63789
摘要

Skin cancer is one of the most common cancers worldwide. Diagnosis relies on visual inspection and dermoscopy followed by biopsy for histopathological confirmation. While the sensitivity of dermoscopy is high, the lower specificity results in 70%-80% of the biopsies being diagnosed as benign lesions on histopathology (false positives on dermoscopy). Reflectance confocal microscopy (RCM) and optical coherence tomography (OCT) imaging can noninvasively guide the diagnosis of skin cancers. RCM visualizes cellular morphology in en-face layers. It has doubled the diagnostic specificity for melanoma and pigmented keratinocytic skin cancers over dermoscopy, halving the number of biopsies of benign lesions. RCM acquired billing codes in the USA and is now being integrated into clinics. However, limitations such as the shallow depth (~200 µm) of imaging, poor contrast for nonpigmented skin lesions, and imaging in en-face layers result in relatively lower specificity for the detection of nonpigmented basal cell carcinoma (BCCs) — superficial BCCs contiguous with the basal cell layer and deeper infiltrative BCCs. In contrast, OCT lacks cellular resolution but images tissue in vertical planes down to a depth of ~1 mm, which allows the detection of both superficial and deeper subtypes of BCCs. Thus, both techniques are essentially complementary. A "multi-modal," combined RCM-OCT device simultaneously images skin lesions in both en-face and vertical modes. It is useful for the diagnosis and management of BCCs (nonsurgical treatment for superficial BCCs vs. surgical treatment for deeper lesions). A marked improvement in specificity is obtained for detecting small, nonpigmented BCCs over RCM alone. RCM and RCM-OCT devices are bringing a major paradigm shift in the diagnosis and management of skin cancers; however, their use is currently limited to academic tertiary care centers and some private clinics. This paper familiarizes clinicians with these devices and their applications, addressing translational barriers into routine clinical workflow.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
ck完成签到,获得积分10
刚刚
英俊的铭应助笨蛋研究生采纳,获得10
1秒前
阿千完成签到 ,获得积分10
2秒前
快乐寄风发布了新的文献求助10
3秒前
theThreeMagi应助科研通管家采纳,获得20
3秒前
今后应助科研通管家采纳,获得10
3秒前
CodeCraft应助科研通管家采纳,获得10
3秒前
4秒前
传奇3应助科研通管家采纳,获得10
4秒前
Cassie应助科研通管家采纳,获得10
4秒前
汉堡包应助科研通管家采纳,获得10
4秒前
香蕉觅云应助科研通管家采纳,获得30
4秒前
66m37发布了新的文献求助10
4秒前
Ava应助科研通管家采纳,获得10
4秒前
高高的戎完成签到,获得积分10
4秒前
kang发布了新的文献求助10
4秒前
英姑应助科研通管家采纳,获得30
4秒前
忆之应助科研通管家采纳,获得10
4秒前
KSDalton应助科研通管家采纳,获得10
4秒前
NexusExplorer应助科研通管家采纳,获得10
5秒前
5秒前
5秒前
乐乐应助科研通管家采纳,获得200
5秒前
5秒前
5秒前
5秒前
zhangzhuopu发布了新的文献求助10
5秒前
睡到自然醒完成签到 ,获得积分10
7秒前
7秒前
萧水白应助神勇一寡采纳,获得10
8秒前
旺旺仙贝完成签到,获得积分10
9秒前
9秒前
10秒前
10秒前
greatsnow发布了新的文献求助10
11秒前
胖大海关注了科研通微信公众号
11秒前
坚强凝安发布了新的文献求助10
12秒前
坦率无剑发布了新的文献求助10
12秒前
郭郭完成签到,获得积分20
12秒前
wweiweili发布了新的文献求助10
12秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
Saponins and sapogenins. IX. Saponins and sapogenins of Luffa aegyptica mill seeds (black variety) 500
Fundamentals of Dispersed Multiphase Flows 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3260545
求助须知:如何正确求助?哪些是违规求助? 2901746
关于积分的说明 8316854
捐赠科研通 2571281
什么是DOI,文献DOI怎么找? 1396969
科研通“疑难数据库(出版商)”最低求助积分说明 653604
邀请新用户注册赠送积分活动 632040