作者
Wenqi Hu,Haitao Zeng,Yanan Shi,Chuanchuan Zhou,Jiana Huang,Lei Jia,Siqi Xu,Xiaoyu Feng,Yanyan Zeng,Tuanlin Xiong,Wenze Huang,Peng Sun,Yajie Chang,Tingting Li,Cong Fang,Keliang Wu,Lingbo Cai,Wuhua Ni,Yan Li,Zhi-Yong Yang,Qiangfeng Cliff Zhang,Ri‐Cheng Chian,Zi‐Jiang Chen,Xiaoyan Liang,Kehkooi Kee
摘要
Abstract The combined use of transcriptome and translatome as indicators of gene expression profiles is usually more accurate than the use of transcriptomes alone, especially in cell types governed by translational regulation, such as mammalian oocytes. Here, we developed a dual-omics methodology that includes both transcriptome and translatome sequencing (T&T-seq) of single-cell oocyte samples, and we used it to characterize the transcriptomes and translatomes during mouse and human oocyte maturation. T&T-seq analysis revealed distinct translational expression patterns between mouse and human oocytes and delineated a sequential gene expression regulation from the cytoplasm to the nucleus during human oocyte maturation. By these means, we also identified a functional role of OOSP2 inducing factor in human oocyte maturation, as human recombinant OOSP2 induced in vitro maturation of human oocytes, which was blocked by anti-OOSP2. Single-oocyte T&T-seq analyses further elucidated that OOSP2 induces specific signaling pathways, including small GTPases, through translational regulation.