Detection and Estimation of Age of Injuries in Fresh Bodies Using Immunohistochemistry

水通道蛋白3 免疫组织化学 H&E染色 病理 染色 伤口愈合 医学 生物 男科 水通道蛋白 生理学 外科
作者
Kokila Ramamurthy,Sandhya Sundaram,Sneha Sivaramakrishnan,Sampath Kumar Paranthaman,Archana B. Balasubramanian,Priyadarshee Pradhan
出处
期刊:American Journal of Forensic Medicine and Pathology [Ovid Technologies (Wolters Kluwer)]
卷期号:44 (4): 293-302 被引量:1
标识
DOI:10.1097/paf.0000000000000867
摘要

Abstract A cross-sectional study of the wound age estimation from the forensic skin wound samples was performed immunohistochemically with a sample size of 40 (n = 40). The samples were segregated according to the appropriate stages of wound healing with the help of hematoxylin-eosin staining. Later, they were subjected to immunohistochemistry staining with anti–AQP3 antibody. Quantification of the expression of AQP3 in the injured and uninjured formalin-fixed skin tissues was done semiquantitatively and manually under 400× magnifications. The AQP3-positive cells were correlated with the duration of injury, and the results were statistically analyzed. More AQP3 expressions were found in the proliferative phase than the inflammatory and maturation phase of wound healing. Neither the diversity in age group nor the sex differentiation showed any specific correlation with the expression of keratinocytic aquaporin cells. Likewise, parameters such as the type of injury, mode of injury, and the postmortem interval also did not show any significant relationship with the expression of the aquaporin positivity. Thus, it is revealed that skin wounds between 5 and 10 days expressed aquaporin cell numbers of more than 300. Hence AQP3 estimation helps in determining the time since injury with a more accuracy.
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