部分
荧光
生物标志物
化学
体外
切除术
荧光寿命成像显微镜
癌症研究
医学
生物化学
病理
外科
立体化学
物理
量子力学
作者
Lijuan Gui,Weiqing Li,Yanping Pan,Zhao Junyuan,Xiangkai Kong,Ji Liu,Kaixuan Zuo,Jun Yan,Yong Ling,Chang‐Chun Ling,Ruixi Li,Zhenwei Yuan
标识
DOI:10.1016/j.snb.2023.134718
摘要
γ-Glutamyltranspeptidase (GGT) is an ectoenzyme anchored on the surface of the cell membrane, and it is closely associated with many diseases. Abnormal expression (>36 U/L) of GGT is involved in the progression of tumors and can act as a biomarker for tumor diagnosis. Here, we presented a series of GGT-activatable fluorescent probes, the P1, the P2 and the P3. By investigating the in vitro properties of the probes, P3 demonstrated the most sensitivity and better water solubility, which benefited from the introduction of the self-elimination moiety of p-aminobenzyl alcohol and the propanesulfonic acid moiety. Importantly, P3 was able to clearly delineate tumor boundaries in live tumor-bearing mice and clinical human liver tissue specimens by spraying in situ, which facilitated accurate resection of tumors under fluorescence guidance. The proposed enzyme-activated fluorescent probe has potential as a surgical resection tool in image-guided surgery.
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