质谱法
化学成像
化学
体内分布
质谱成像
分析化学(期刊)
色谱法
计算机科学
高光谱成像
人工智能
生物化学
体外
作者
Natalie A. Belsey,Alex Dexter,Jean‐Luc Vorng,Dimitrios Tsikritsis,Chelsea J. Nikula,Teresa Murta,Mariavitalia Tiddia,Junting Zhang,Elzbieta Gurdak,Gustavo F. Trindade,Ian S. Gilmore,Leanne Page,Clive Roper,Richard H. Guy,Mila Boncheva Bettex
标识
DOI:10.1016/j.jconrel.2023.10.026
摘要
A correlative methodology for label-free chemical imaging of soft tissue has been developed, combining non-linear optical spectroscopies and mass spectrometry to achieve sub-micron spatial resolution and critically improved drug detection sensitivity. The approach was applied to visualise the kinetics of drug reservoir formation within human skin following in vitro topical treatment with a commercial diclofenac gel. Non-destructive optical spectroscopic techniques, namely stimulated Raman scattering, second harmonic generation and two photon fluorescence microscopies, were used to provide chemical and structural contrast. The same tissue sections were subsequently analysed by secondary ion mass spectrometry, which offered higher sensitivity for diclofenac detection throughout the epidermis and dermis. A method was developed to combine the optical and mass spectrometric datasets using image registration techniques. The label-free, high-resolution visualisation of tissue structure coupled with sensitive chemical detection offers a powerful method for drug biodistribution studies in the skin that impact directly on topical pharmaceutical product development.
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