Sandwich Biomimetic Scaffold Based Tendon Stem/Progenitor Cell Alignment in a 3D Microenvironment for Functional Tendon Regeneration

脚手架 材料科学 再生(生物学) 肌腱 祖细胞 跟腱 生物医学工程 干细胞 细胞生物学 解剖 生物 医学
作者
Sihao Li,Yuan Sun,Yazhou Chen,Jinwei Lu,Guangyao Jiang,Kang Yu,Yifan Wu,Yufei Mao,Hao Jin,Jikui Luo,Shurong Dong,Bin Hu,Yi Ding,An Liu,Yu Shen,Gang Feng,Shigui Yan,Yong He,Ruijian Yan
出处
期刊:ACS Applied Materials & Interfaces [American Chemical Society]
卷期号:15 (3): 4652-4667 被引量:18
标识
DOI:10.1021/acsami.2c16584
摘要

Tendon injuries are some of the most commonly diagnosed musculoskeletal diseases. Tendon regeneration is sensitive to the topology of the substitute as it affects the cellular microenvironment and homeostasis. To bionic in vivo three-dimensional (3D) aligned microenvironment, an ordered 3D sandwich model was used to investigate the cell response in the tendon. First, high-resolution 3D printing provided parallel-grooved topographical cues on the hydrogel surface. Then the cells were seeded on its surface to acquire a 2D model. Afterward, an additional hydrogel coating layer was applied to the cells to create the 3D model. The interaction between cells and order structures in three-dimensions is yet to be explored. The study found that the tendon stem/progenitor cells (TSPCs) still maintain their ordering growth in the 3D model as in the 2D model. The study also found that the 3D-aligned TSPCs exhibited enhanced tenogenic differentiation through the PI3K-AKT signaling pathway and presented a less inflammatory phenotype than those in the 2D model. The in vivo implantation of such a 3D-aligned TSPC composite promoted tendon regeneration and mitigated heterotopic ossification in an Achilles defect model. These findings demonstrated that 3D-aligned TSPCs within a biomimetic topology environment are promising for functional tendon regeneration.
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