清脆的
环介导等温扩增
桑格测序
放大器
计算生物学
管道(软件)
计算机科学
回文
纳米孔测序
生物
病毒学
突变
遗传学
聚合酶链反应
基因组
基因
操作系统
DNA
作者
Gabriel Lamothe,Julie Carbonneau,Charles Joly Beauparlant,Thierry Vincent,Patrik Quessy,Anthony Guedon,Gary P. Kobinger,Jean-François Lemay,Guy Boivin,Arnaud Droit,Nathalie Turgeon,Jacques P. Tremblay
出处
期刊:The CRISPR journal
[Mary Ann Liebert]
日期:2023-06-21
标识
DOI:10.1089/crispr.2023.0007
摘要
The worldwide proliferation of the SARS-CoV-2 virus in the past 3 years has allowed the virus to accumulate numerous mutations. Dangerous lineages have emerged one after another, each leading to a new wave of the pandemic. In this study, we have developed the THRASOS pipeline to rapidly discover lineage-specific mutation signatures and thus advise the development of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-based diagnostic tests. We also optimized a strategy to modify loop-mediated isothermal amplification amplicons for downstream use with Cas12 and Cas13 for future multiplexing. The close ancestry of the BA.1 and BA.2 variants of SARS-CoV-2 (Omicron) made these excellent candidates for the development of a first test using this workflow. With a quick turnaround time and low requirements for laboratory equipment, the test we have created is ideally suited for settings such as mobile clinics lacking equipment such as Next-Generation Sequencers or Sanger Sequencers and the personnel to run these devices.
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