生物
烟草
液泡蛋白分选
突变体
酿酒酵母
拟南芥
蛋白质靶向
遗传学
细胞生物学
遗传筛选
内体
位置克隆
基因
拟南芥
膜蛋白
膜
细胞内
作者
Keke Kong,Mengge Xu,Xu Zhang,Wenhuan Lv,Peiyun Lv,Naheeda Begum,Bingqiang Liu,Bin Liu,Tuanjie Zhao
出处
期刊:Plant Science
[Elsevier]
日期:2023-06-01
卷期号:331: 111677-111677
被引量:3
标识
DOI:10.1016/j.plantsci.2023.111677
摘要
Vacuolar Protein Sorting 8 (Vps8) protein is a specific subunit of the class C core vacuole/endosome tethering (CORVET) complex that plays a key role in endosomal trafficking in yeast (Saccharomyces cerevisiae). However, its functions remain largely unclear in plant vegetative growth. Here, we identified a soybean (Glycine max) T4219 mutant characterized with compact plant architecture. Map-based cloning targeted to a candidate gene GmVPS8a (Glyma.07g049700) and further found that two nucleotides deletion in the first exon of GmVPS8a causes a premature termination of the encoded protein in the T4219 mutant. Its functions were validated by CRISPR/Cas9-engineered mutation in the GmVPS8a gene that recapitulated the T4219 mutant phenotypes. Furthermore, NbVPS8a-silenced tobacco (Nicotiana benthamiana) plants exhibited similar phenotypes to the T4219 mutant, suggesting its conserved roles in plant growth. The GmVPS8a is widely expressed in multiple organs and its protein interacts with GmAra6a and GmRab5a. Combined analysis of transcriptomic and proteomic data revealed that dysfunction of GmVPS8a mainly affects pathways on auxin signal transduction, sugar transport and metabolism, and lipid metabolism. Collectively, our work reveals the function of GmVPS8a in plant architecture, which may extend a new way for genetic improvement of ideal plant-architecture breeding in soybean and other crops.
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