生物
脂肪组织
基质血管部分
干细胞
间质细胞
差速器(机械装置)
细胞生物学
内分泌学
内科学
癌症研究
医学
航空航天工程
工程类
作者
Hannah M Carr,Malke Asaad,Yewen Wu,Cynthia D. Branch-Brooks,Qixu Zhang,Peiman Hematti,Summer E. Hanson
出处
期刊:Stem Cells and Development
[Mary Ann Liebert]
日期:2024-08-09
标识
DOI:10.1089/scd.2024.0071
摘要
There are multiple methods to prepare lipoaspirate for autologous fat transfer; however, graft retention remains unpredictable. The purpose of this study was to compare the cellular and protein composition of adipose grafts and the stromal vascular fraction (SVF) resulting from three common techniques to prepare adipose grafts. Adipose grafts were harvested from healthy donors and processed via three techniques: centrifugation (C), a single-filter (SF) device, and a double-filtration (DF) system. Part of each graft was analyzed or further processed to isolate the SVF. Cell viability, surface markers, cytokine, and growth factors were compared between the graft and SVF as well as adipose-derived stem cells (ASCs). Overall, we found variations across the three processing techniques and among the graft components (ASCs, SVF, and fat). Cell viability within the grafts was similar (94.6%, 92.3%, and 93.6%;
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