Establishment of Genetic Transformation System in Lilium pumilum and Functional Analysis of LpNAC6 on Abiotic Stress

Lilium pumilum is widely distributed in northeast Asia. It exhibits strong resistance and possesses high ornamental value. However, it currently lacks an efficient and stable transformation system. Therefore, we aimed to establish an effective genetic transformation system using the Agrobacterium-mediated method for L. pumilum, enabling gene transfer into the plant for gene function research and genetic engineering breeding. Our genetic transformation system achieved a maximum transformation efficiency of 7.25% under specific conditions: a kanamycin (Kana) concentration of 120mg/L, 3 days of pre-cultivation, an A. tumefaciens concentration of 0.7 OD