铜绿假单胞菌
一致性
微生物学
细菌
生物
肠杆菌科
大肠杆菌
遗传学
基因
作者
Braulio Josué Mendez-Sotelo,Luis Esaú López-Jácome,Claudia Adriana Colín‐Castro,Melissa Hernández-Durán,María Guadalupe Martínez-Zavaleta,Frida Rivera,Consuelo Velázquez-Acosta,Ana Patricia Rodríguez-Zulueta,María del Rayo Morfin-Otero,Rafael Franco‐Cendejas
出处
期刊:Antibiotics
[MDPI AG]
日期:2023-01-06
卷期号:12 (1): 96-96
被引量:7
标识
DOI:10.3390/antibiotics12010096
摘要
The identification of carbapenemase-producing Enterobacterales and Pseudomonas aeruginosa is important for treating and controlling hospital infections. The recommended methods for their identification require a long waiting time, technical training, and expertise. Lateral flow immunoassays such as NG-Test CARBA 5® overcome these needs. We analyzed 84 clinical isolates of carbapenem-resistant Enterobacterales and P. aeruginosa from four different hospitals in a two-year period. Antimicrobial resistance patterns were confirmed with the broth dilution method. Evaluation of KPC, VIM, NDM, IMP, and OXA-48-like enzymes was performed and compared to NG-Test CARBA 5 and phenotypic assays. Enterobacterales represented 69% of isolates and P. aeruginosa represented 31%. Carbapenemase-producing strains were 51 (88%) of Enterobacterales and 23 (88.4%) of P. aeruginosa; 20 (34%) and 23 (88%) were Class B ß-lactamases, respectively. The NG-Test CARBA 5® assay for Enterobacterales showed high sensitivity (98%), specificity (100%), and PPV (100%); however, it did not for P. aeruginosa. The Kappa concordance coefficient was 0.92 for Enterobacterales and 0.52 for P. aeruginosa. NG-Test CARBA 5® is a fast and easy-to-use assay. In Enterobacterales, we found excellent agreement in our comparison with molecular tests. Despite the low agreement in P. aeruginosa, we suggest that this test could be used as a complementary tool.
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