Determinants of the microbiome spatial variability in chronic rhinosinusitis

窦(植物学) 微生物群 慢性鼻-鼻窦炎 医学 鼻腔 采样(信号处理) 上颌窦 生物 解剖 外科 生物信息学 生态学 滤波器(信号处理) 计算机科学 计算机视觉
作者
Joanna Szaleniec,Valentyn Bezshapkin,Agnieszka Krawczyk,Katarzyna Kopera,Barbara Zapała,Tomasz Gosiewski,Tomasz Kościółek
出处
期刊:Rhinology [European Rhinologic Society]
被引量:2
标识
DOI:10.4193/rhin22.423
摘要

BACKGROUND: The sinus microbiome in patients with chronic rhinosinusitis (CRS) is considered homogenous across the sinonasal cavity. The middle nasal meatus is the recommended sampling site for 16S rRNA sequencing. However, individuals with unusually high between-site variability between the middle meatus and the sinuses were identified in previous studies. This study aimed to identify which factors determine increased microbial heterogeneity between sampling sites in the sinuses. METHODOLOGY: In this cross-sectional study samples for 16S rRNA sequencing were obtained from the middle meatus, the maxillary and the frontal sinus in 50 patients with CRS. The microbiome diversity between sampling sites was analysed in relation to the size of the sinus ostia and clinical metadata. RESULTS: In approximately 15% of study participants, the differences between sampling sites within one patient were greater than between the patient and other individuals. Contrary to a popular hypothesis, obstruction of the sinus ostium resulted in decreased dissimilarity between the sinus and the middle meatus. The dissimilarity between the sampling sites was patient-specific: greater between-sinus differences were associated with greater meatus-sinus differences, regardless of the drainage pathway patency. Decreased spatial variability was observed in patients with nasal polyps and extensive mucosal changes in the sinuses. CONCLUSIONS: Sampling from the middle meatus is not universally representative of the sinus microbiome. The differences between sites cannot be predicted from the patency of communication pathways between them.
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