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Establishment of a multi-strategy platform for quality control and quality markers screen of Mailuoshutong pill

芍药苷 毛花素 中医药 药理学 化学 医学 内科学 芒柄花素 色谱法 高效液相色谱法 大豆黄酮 替代医学 病理 染料木素
作者
Yaojuan Chu,Xiangyu Zhang,Li-hua Zuo,Xiaobao Wang,Yingying Shi,Liwei Liu,Lin Zhou,Jian Kang,Bing Li,Wenbo Cheng,Shu-zhang Du,Zhi Sun
出处
期刊:Journal of Pharmaceutical and Biomedical Analysis [Elsevier]
卷期号:: 116070-116070
标识
DOI:10.1016/j.jpba.2024.116070
摘要

Thromboangiitis obliterans (TAO) is a non-atherosclerotic segmental inflammatory occlusive disease with a high recurrence rate, high disability rate, difficulty to cure, and poor prognosis. It has been clinically proven that Mailuoshutong pill (MLSTP) is an effective traditional Chinese medicine for treating TAO. As MLSTP contains hundreds of chemical components, the quality control of which is a challenge in the development of reliable quality evaluation metrics. This study aimed to evaluate the quality uniformity of MLSTP by establishing a multi-strategy platform. In the present study, the key targets and signaling pathways of MLSTP treating TAO were predicted by network pharmacology. It was further shown by in vivo validation experiments that MLSTP exerted therapeutic effects on TAO by modulating the PI3K-AKT signaling pathway, VEGF signaling pathway, and HIF-1 signaling pathway. In addition, UPLC fingerprints of MLSTP were established and screened for potential Q-markers of MLSTP in combination with network pharmacology results. Six components, including chlorogenic acid, liquiritin, paeoniflorin, calycosin-7-glucoside, berberine, and formononetin, were selected as potential quality markers (Q-markers) in MLSTP. Finally, the quantitative analysis of multi-components by single marker (QAMS) method was established to quantitatively analyze the six potential Q-markers, and the results were consistent with those obtained by the external standard method (ESM). Taken together, the multi-strategy platform established in this study would be conducive to the Q-markers screening and quality control of MLSTP, improving the quality standard of MLSTP and providing favorable assurance for the clinical management of TAO.
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