核酸
DNA
锁核酸
化学
核酸检测
计算生物学
生物化学
组合化学
生物
寡核苷酸
作者
Yuting Shuai,Yi‐Hsu Ju,Yuanhang Li,Dini Ma,Lan Jiang,Jingyu Zhang,Gao‐Yi Tan,Xueting Liu,Shenlin Wang,Lixin Zhang,Liu Guang
标识
DOI:10.1016/j.synbio.2023.02.002
摘要
Nucleic acid detection plays a key role in diverse diagnosis and disease control. Currently available nucleic acid detection techniques are challenged by trade-offs among speed, simplicity, precision and cost. Here, we described a novel method, designated SENSOR (Sulfur DNA mediated nucleic acid sensing platform), for rapid nucleic acid detection. SENSOR was developed from phosphorothioate (PT)-DNA and sulfur binding domain (SBD) which specifically binds double-stranded PT-modified DNA. SENSOR utilizes PT-DNA oligo and SBD as targeting module, which is linked with split luciferase reporter to generate luminescence signal within 10 min. We tested detection on synthesized nucleic acid and COVID-19 pseudovirus, achieving attomolar sensitivity combined with an amplification procedure. Single nucleotide polymorphisms (SNP) could also be discriminated. Indicating SENSOR a new promising nucleic acid detection technique.
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