川地68
子宫内膜
CD3型
CD8型
免疫系统
妇科
CD14型
黄体期
男科
胚胎
内科学
医学
生物
免疫学
免疫组织化学
卵泡期
细胞生物学
作者
Rumiana Ganeva,Dimitar Parvanov,Nina Vidolova,M Ruseva,Maria Handzhiyska,Katarina Arsov,Ivon Decheva,Dimitar Metodiev,Veselina Moskova‐Doumanova,Georgi Stamenov
标识
DOI:10.1016/j.jri.2023.103816
摘要
The objective of this study was to compare the endometrial immune cells quantities and ratios during the mid-luteal phase between women with recurrent implantation failure (RIF) with successful and unsuccessful embryo implantation. For this purpose, endometrial biopsies from 116 women aged between 29 and 46 with history of RIF undergoing Assisted Reproductive Technology (ART) without endometrial pathologies were immunohistochemically stained for CD3 + T-cells, CD4 + T-helpers, CD8 + T-killers, CD14 + monocytes, CD68 + macrophages, CD56 + NK cells and CD79α+ B-cells. Endometrial immune cells quantities and ratios were compared based on the embryo implantation outcome in the subsequent embryo transfer cycle. Spearman correlation analysis and Mann-Whitney U test were used to analyse the obtained data. Patients who experienced successful implantation at the subsequent cycle had significantly lower percentage of CD3 + T cells, and higher ratios of CD4 + /CD8 + , CD4 + /CD3 + and CD68 + /CD3 + than the patients who experienced another failure in implantation. In addition, the ratios of CD3 + /CD14 + , CD79α+ /CD14 + and CD56 + /CD14 + were significantly lower in the successful implantation group than that in the unsuccessful one. A cut off value of CD68 + /CD3 + ratio higher than 0.85 (AUC 0.67, 95% CI 0.56-0.79), CD4 + /CD3 + ratio higher than 0.19 (AUC 0.67, 95% CI 0.56-0.79) and CD4 + /CD8 + ratio higher than 0.43 (AUC 0.62, 95% CI 0.50-0.73) could be predictive factors for successful implantation in RIF patients. Knowledge on the immune cell composition could assist in the evaluation of the endometrial receptivity in RIF patients.
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