Synthesis of quantum dot encoded multicolour nanobeads for the ultrasensitive and multiplex immunochromatographic detection of neonicotinoid insecticides

化学 噻虫嗪 色谱法 检出限 噻虫胺 多路复用 分子印迹聚合物 新烟碱 益达胺 杀虫剂 生物化学 选择性 生物 农学 生物信息学 催化作用
作者
Hui Cao,Shujie Cao,Yiyi Han,Weiyi Zhang,Ziqi Wei,Tai Ye,Min Yuan,Jinsong Yu,Xiuxiu Wu,Liling Hao,Fengqin Yin,Dongsheng Feng,Fei Xu
出处
期刊:Sensors and Actuators B-chemical [Elsevier BV]
卷期号:361: 131671-131671 被引量:14
标识
DOI:10.1016/j.snb.2022.131671
摘要

In this study, a novel fluorescent immunochromatographic assay (ICA) strip based on quantum dot encoded multicolour nanobead (QB) was developed to detect three neonicotinoid insecticides in the vegetable samples simultaneously. The results showed that three QBs with red-, yellow- and green-emitting were successfully synthesized by embedding different ratios of two kinds of hydrophobic CdSe/ZnS QDs (λ em = 565 nm and λ em = 620 nm) into the polymer matrix. The obtained three QBs were further used to label the monoclonal antibodies (mAbs) specific to imidacloprid (IMI), clothianidin (CLT) and thiamethoxam (TMX). The resultant QB@mAbs nanoprobes, including QB red @IMI mAbs, QB yellow @CLT mAbs and QB green @TMX mAbs, presented excellent monodispersity with distinguishable emission colours. Furthermore, the fabricated QB-ICA strip based on QB@mAbs nanoprobes achieved a more sensitive simultaneous detection for three neonicotinoids with the detection limits of 14.58 ng/mL for IMI, 0.27 ng/mL for CLT, and 0.36 ng/mL for TMX, respectively. This was better than most of the conventional based enzyme-linked immunosorbent assay (ELISA). Moreover, the QB-based ICA strips also exhibited good stability and specificity for the multiplex detection of IMI, CLT, and TMX. In the spiked Chinese cabbage samples, the recovery of IMI, CLT, and TMX ranged from 72.08% to 98.70%, with RSD values ranging from 3.60% to 13.09%. In brief, the great performance of the developed QB-ICA approach demonstrates its potential for simultaneous and quantitative neonicotinoids detection in agricultural products. • The mAbs specific to IMI, CLT and TMX were labelled by quantum dot encoded multicolour nanobead (QB). • Three QB@mAbs were used to fabricate the QB-ICA system for the detection of IMI, CLT and TMX. • The QB-ICA strip has a high sensitivity for IMI, CLT and TMX detection with a LOD of 14.58, 0.27 and 0.36 ng/mL. • This work achieves the simultaneous detection of three neonicotinoids via ICA.
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