Lysosome-targeting benzothiazole-based fluorescent probe for imaging viscosity and hypochlorite levels in living cells and zebrafish

Viscosity and hypochlorite (OCl-) play an important role in biological activities and may cause negative effects at abnormal levels. In this study, a novel lysosome-target fluorescent probe BDHA was obtained based on a benzothiazole derivative. Probe BDHA showed linear ranges of detection for viscosity from 1.62 cP to 851.6 cP with a fluorescent turn-on response. It can also be used as a sensor for OCl- with a turn-off response and showed a good linear range from 0 to 390 μM, with the detection limit calculated to be 2.8 μM. Moreover, BDHA can also be used to image viscosity and OCl- levels in HeLa cells and zebrafish, owing to its excellent optical properties.