神经保护
免疫印迹
神经营养因子
多巴胺能
车站3
磷酸化
神经营养素
帕金森病
化学
肿瘤坏死因子α
药理学
细胞生物学
生物
神经科学
医学
多巴胺
内科学
免疫学
生物化学
疾病
受体
基因
作者
Xueping Yang,Qingyun Yv,Fanlong Ye,Sheng Chen,Zhang He,Wenwei Li,Fang Dong
标识
DOI:10.3389/fphar.2022.848813
摘要
Echinacoside (ECH), the major active constituent of Cistanche deserticola, was found to exert neuroprotection through neurotrophic and anti-inflammatory functions in Parkinson's disease (PD) models. However, a clear intermediate molecule or pathway that unifies these two effects has to be found. In this study, our results demonstrate that ECH can protect DA neurons in PD mice with Western blot and immunohistochemistry staining. The quantitative real-time polymerase chain reaction was adapted to confirm its anti-inflammatory function with decreased cytokines (interleukin- (IL-) 6, IL-1β, and TNF-α) in PD mice and LPS-induced BV2 cells. Further studies found that ECH inhibited the IL-6/JAK2/STAT3 pathway and decreased phosphorylation of STAT3 on tyr705 by Western blot. It can also increase p-STAT3 (ser727) and brain-derived neurotrophic factor (BDNF) expression in PD mice and LPS-induced BV2 cells. This study revealed that ECH exerts neurotrophic and anti-inflammatory effects by regulating the IL-6/JAK2/STAT3 pathway and the phosphorylation of STAT3, promoting the mutually beneficial influence of the two effects to maximize its neuroprotective function.
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