雌激素受体α
间充质干细胞
雌激素受体
阿尔法(金融)
骨髓
生物
细胞生物学
雌激素受体
干细胞
干细胞移植修复关节软骨
癌症研究
成体干细胞
医学
免疫学
细胞分化
基因
生物化学
遗传学
癌症
乳腺癌
结构效度
护理部
患者满意度
作者
Wang Qi,Jinhua Yu,Huihong Zhai,Qing-tao Zhao,Jinwu Chen,Lei Shu,Deqiang Li,Dayong Liu,Chen Dong,Yin Ding
标识
DOI:10.1016/j.bbrc.2006.06.070
摘要
Estrogen responsiveness of bone formation is mediated by the estrogen receptor alpha (ERα) in osteoblastic lineage. As osteoblasts arise from the multipotent bone marrow stromal (mesenchymal) cells, this study was undertaken to observe the ERα in primary female adult rat bone marrow mesenchymal stem cells (BMSCs). The ERα was localized using immunocytochemical analysis in identified primary BMSCs. Then, using real-time PCR analysis, we measured the expression of ERα messenger RNA (mRNA) in BMSCs. ERα transcripts showed different trends between untreated cultures (control group) and osteogenic-induced cultures (treated group). In the control group, ERα mRNA climbed at peak levels at a confluence stage and decreased until day 20, whereas, in the treated group, the ERα mRNA kept climbing from a low level until day 20. Thus, the observed developmental expression of ERα mRNA correlates with progressive BMSCs growth and osteogenic differentiation and BMSCs may be a primary target cell for estrogen in maintaining bone formation.
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