屎肠球菌
金标准(测试)
粪肠球菌
肠球菌
万古霉素
微生物学
多路复用
多重聚合酶链反应
聚合酶链反应
生物
医学
细菌
抗生素
金黄色葡萄球菌
内科学
基因
生物信息学
生物化学
遗传学
作者
Dona Benadof,Marcela San Martín,J Aguirre,Liliana Paredes,Rodrigo Malig,Francisco S. Melo,Gaëlle Lehouque
标识
DOI:10.1016/j.jinf.2010.02.011
摘要
Objectives This study describes the diagnostic performance of a recently available multiplex PCR-based kit for the simultaneous detection and identification of Enterococcus faecium, Enterococcus faecalis, vanA, vanB, vanC1 and vanC2/C3 genes, directly from rectal swabs constituting the most complete existing molecular assay currently available. Methods The diagnostic performance of this assay was evaluated by a multicenter study involving three independent public hospitals and consisted in the analysis of 187 rectal swabs from patients at high risk for vancomycin-resistant enterococci colonization. Results When bacteria culture was used as the gold standard, the sensitivity, specificity, positive and negative predicted values for the assay were 96.8%, 76.0%, 67.7% and 97.9%, respectively. When a composite reference standard consisting of culture and DNA sequencing of PCR products was used as the gold standard, the sensitivity, specificity, positive and negative predicted values for the PCR-based assay were 97.8%, 96.9%, 96.7% and 97.9%, respectively. Conclusions Based on these results, we conclude that this assay is considerably more sensitive than traditional microbiological methods for detecting vancomycin-resistant enterococci from rectal swabs. It is also much faster than culture. We believe that the implementation of this assay in routine clinical laboratories could help to reduce hospital-acquired vancomycin-resistant enterococci infections.
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