T细胞受体
逆转录病毒
转基因
生物
转基因小鼠
混乱
T细胞
基因剔除小鼠
受体
细胞生物学
分子生物学
基因
计算生物学
免疫学
遗传学
免疫系统
心理学
精神分析
作者
Jeff Holst,Andrea L. Szymczak-Workman,Kate M. Vignali,Amanda R. Burton,Creg J. Workman,Dario A.A. Vignali
出处
期刊:Nature Protocols
[Springer Nature]
日期:2006-06-01
卷期号:1 (1): 406-417
被引量:257
标识
DOI:10.1038/nprot.2006.61
摘要
T-cell receptor (TCR) transgenic (Tg) mice have revolutionized our understanding of many aspects of T-cell biology. Whereas they provide an almost unlimited source of T cells with a single specificity, breeding them onto different backgrounds and/or new knockout/knock-in mouse models is often time-consuming (6 months to several years), which can make the process costly and can significantly delay research. This protocol describes a new method for expressing defined TCR-alpha and TCR-beta proteins from a single 2A peptide-linked multicistronic retroviral vector in mice, using retrovirus-mediated stem cell gene transfer. We refer to these as 'retrogenic' (Rg) mice ('retro' from retrovirus and 'genic' from Tg) to avoid confusion with traditional transgenic mice. We have successfully used this approach to express over 50 different TCRs on several different mouse backgrounds in as little as 6 weeks.
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