DNA甲基化
表观遗传学
亚硫酸氢盐测序
生物
亚硫酸氢盐
CpG站点
DNA
计算生物学
甲基化
照明菌甲基化试验
甲基化DNA免疫沉淀
DNA测序
基因组
遗传学
分子生物学
基因
基因表达
作者
Sébastien A. Smallwood,Heather Lee,Christof Angermueller,Felix Krueger,Heba Saadeh,Julian R. Peat,Simon Andrews,Oliver Stegle,Wolf Reik,Gavin Kelsey
出处
期刊:Nature Methods
[Springer Nature]
日期:2014-07-20
卷期号:11 (8): 817-820
被引量:988
摘要
Single-cell bisulfite sequencing (scBS-seq) allows robust DNA methylation analysis in rare cells and heterogeneous populations. We report a single-cell bisulfite sequencing (scBS-seq) method that can be used to accurately measure DNA methylation at up to 48.4% of CpG sites. Embryonic stem cells grown in serum or in 2i medium displayed epigenetic heterogeneity, with '2i-like' cells present in serum culture. Integration of 12 individual mouse oocyte datasets largely recapitulated the whole DNA methylome, which makes scBS-seq a versatile tool to explore DNA methylation in rare cells and heterogeneous populations.
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