癌胚抗原
胸苷激酶
单纯疱疹病毒
转染
更昔洛韦
遗传增强
赫拉
分子生物学
细胞培养
癌症研究
病毒学
生物
A549电池
医学
病毒
肺癌
基因
癌症
病理
内科学
人巨细胞病毒
生物化学
遗传学
作者
Tadashi Osaki,Yoshiro Tanio,Isao Tachibana,Shigeto Hosoe,Toru Kumagai,Ichiro Kawase,Shinzo Oikawa,Tadamitsu Kishimoto
出处
期刊:Lung Cancer
[Elsevier]
日期:1995-06-01
卷期号:12 (3): 326-326
被引量:75
标识
DOI:10.1016/0169-5002(95)99039-k
摘要
A carcinoembryonic antigen (CEA)-producing human lung cancer cell line (A549), a nonproducing human lung cancer cell line (CADO-LC9), and a human uterine cervical cancer (HeLa) were transfected with the herpes simplex virus thymidine kinase (HSV-TK) gene regulated by 445 nucleotides upstream from the translational start of CEA gene. Fifty % growth inhibitory concentration of ganciclovir (GCV) was 0.57 micron for HSV-TK-transfected A549; relative sensitivity to GCV was more than 1000 times higher compared to the 50% growth inhibitory concentration of the parental cell line. Both CADO-LC9 and HeLa transfected with HSV-TK were still resistant to GCV. There was no difference in either morphology or doubling time between HSV-TK-transfected and parental clones. Injections (i.p.) of GCV resulted in significant regression of HSV-TK-transfected A549 tumors in nude mice. These data show the possibility of gene therapy using the cell type-specific promoter of CEA gene against CEA-producing adenocarcinoma of the lung.
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