Dysbiosis of the faecal microbiota in patients with Crohn's disease and their unaffected relatives

瘤胃球菌 失调 普氏粪杆菌 肠道菌群 双歧杆菌 生物 微生物学 维管菌 医学 乳酸菌 免疫学 内科学 细菌 遗传学 链球菌
作者
Marie Joossens,Geert Huys,Margo Cnockaert,Vicky De Preter,Kristin Verbeke,Paul Rutgeerts,Peter Vandamme,Séverine Vermeire
出处
期刊:Gut [BMJ]
卷期号:60 (5): 631-637 被引量:978
标识
DOI:10.1136/gut.2010.223263
摘要

Background and aims

A general dysbiosis of the intestinal microbiota has been established in patients with Crohn9s disease (CD), but a systematic characterisation of this dysbiosis is lacking. Therefore the composition of the predominant faecal microbiota of patients with CD was studied in comparison with the predominant composition in unaffected controls. Whether dysbiosis is present in relatives of patients CD was also examined.

Methods

Focusing on families with at least three members affected with CD, faecal samples of 68 patients with CD, 84 of their unaffected relatives and 55 matched controls were subjected to community fingerprinting of the predominant microbiota using denaturing gradient gel electrophoresis (DGGE). To analyse the DGGE profiles, BioNumerics software and non-parametric statistical analyses (SPSS V.17.0) were used. Observed differences in the predominant microbiota were subsequently confirmed and quantified with real-time PCR.

Results

Five bacterial species characterised dysbiosis in CD, namely a decrease in Dialister invisus (p=0.04), an uncharacterised species of Clostridium cluster XIVa (p=0.03), Faecalibacterium prausnitzii (p<1.3×10−5) and Bifidobacterium adolescentis (p=5.4×10−6), and an increase in Ruminococcus gnavus (p=2.1×10−7). Unaffected relatives of patients with CD had less Collinsella aerofaciens (p=0.004) and a member of the Escherichia coli–Shigella group (p=0.01) and more Ruminococcus torques (p=0.02) in their predominant microbiota as compared with healthy subjects.

Conclusion

Unaffected relatives of patients with CD have a different composition of their microbiota compared with healthy controls. This dysbiosis is not characterised by lack of butyrate producing-bacteria as observed in CD but suggests a role for microorganisms with mucin degradation capacity.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Jason完成签到,获得积分10
刚刚
害羞凤灵完成签到,获得积分10
刚刚
芳芳完成签到,获得积分10
1秒前
风起枫落完成签到 ,获得积分10
1秒前
xkhxh完成签到 ,获得积分10
2秒前
zzq778发布了新的文献求助10
2秒前
小马甲应助双儿采纳,获得10
3秒前
江南烟雨如笙完成签到 ,获得积分10
4秒前
王洋应助枕星采纳,获得10
7秒前
笨笨寒天完成签到,获得积分10
7秒前
Hello应助zzq778采纳,获得10
7秒前
8秒前
铜豌豆完成签到 ,获得积分10
8秒前
稞小弟完成签到,获得积分10
8秒前
9秒前
11秒前
11秒前
zzzz发布了新的文献求助10
12秒前
小马完成签到,获得积分10
14秒前
14秒前
一朵云完成签到,获得积分10
15秒前
JSY发布了新的文献求助30
16秒前
浩铭完成签到,获得积分10
17秒前
Iven发布了新的文献求助10
17秒前
19秒前
21秒前
冷酷的天晴完成签到,获得积分10
21秒前
ysy完成签到,获得积分10
22秒前
22秒前
yum完成签到,获得积分20
23秒前
Ava应助专注的白柏采纳,获得10
23秒前
何如当初莫相识完成签到,获得积分10
23秒前
24秒前
落后从阳发布了新的文献求助10
26秒前
372925abc完成签到,获得积分10
26秒前
27秒前
清秀颜演完成签到,获得积分10
27秒前
carbonhan完成签到,获得积分10
28秒前
sh131完成签到,获得积分10
28秒前
复杂念梦完成签到 ,获得积分10
29秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
Research on Disturbance Rejection Control Algorithm for Aerial Operation Robots 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038368
求助须知:如何正确求助?哪些是违规求助? 3576068
关于积分的说明 11374313
捐赠科研通 3305780
什么是DOI,文献DOI怎么找? 1819322
邀请新用户注册赠送积分活动 892672
科研通“疑难数据库(出版商)”最低求助积分说明 815029