Correlation between antitumor activity, molecular weight, and conformation of lentinan

香菇多糖 化学 水溶液 三螺旋 色谱法 体内 回转半径 水动力半径 大小排阻色谱法 粘度计 多糖 结晶学 核化学 立体化学 有机化学 粘度 生物技术 胶束 物理 生物 聚合物 量子力学
作者
Lina Zhang,Xuelian Li,Xiaojuan Xu,Fanbo Zeng
出处
期刊:Carbohydrate Research [Elsevier BV]
卷期号:340 (8): 1515-1521 被引量:293
标识
DOI:10.1016/j.carres.2005.02.032
摘要

A (1→3)-β-d-glucan having (1→6) branching (L-FV-IB) from Lentinus edodes in water was degraded into seven fractions of different molecular weights by ultrasonic irradiation, and each was further fractionated into three parts, by precipitation from water into acetone at room temperature. The weight-average molecular weight (Mw), radius of gyration (z1/2), and intrinsic viscosity ([η]) of lentinan and its fractions in 0.9% NaCl aqueous solution and dimethyl sulfoxide (Me2SO) were determined by size-exclusion chromatography combined with multi-angle laser light scattering (SEC-LLS), LLS, and viscometry. Analysis of Mw, [η], and z1/2 in terms of known theory for worm-like chains yielded 2240 ± 100 nm−1, and 100 ± 10 nm for molar mass per unit contour length (ML), and persistence length (q), respectively, corresponding with theoretical data for triple-helical chains. The [α]D of lentinan in water–Me2SO mixtures indicated an order–disorder transition. The results indicated that lentinan exists as a triple helix in 0.9% NaCl aqueous solution and as a single flexible chain in Me2SO. Assays in vivo and in vitro against the growth of Sarcoma 180 solid tumor as well as the colorimetric 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method for lentinan showed that the triple-helix sample exhibited a relatively high inhibition ratio. Interestingly, the triple-helix lentinan with Mw of 1.49 × 106 exhibited the highest antitumor activity in vivo, having an inhibition ratio (ξ) of 49.5%, close to that of 5-fluorouracil (ξ = 50.5%), whereas the bioactivity (ξ = 12.3%) of its single flexible chains almost disappeared. The triple-helix conformation plays an important role in enhancing the antitumor effects of lentinan.
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