Cotton Major Latex Protein 28 Functions as a Positive Regulator of the Ethylene Responsive Factor 6 in Defense against Verticillium dahliae

大丽花黄萎病 生物 茉莉酸 基因敲除 异位表达 基因沉默 转录因子 切尔独铵 细胞生物学 基因表达 WRKY蛋白质结构域 植物对草食的防御 水杨酸 基因 植物 遗传学 转录组
作者
Chun-Lin Yang,Shan Liang,Haiyun Wang,Libo Han,Fuxin Wang,Huanqing Cheng,Xiaomin Wu,Zhan-Liang Qu,Jiahe Wu,Gui-Xian Xia
出处
期刊:Molecular Plant [Elsevier]
卷期号:8 (3): 399-411 被引量:126
标识
DOI:10.1016/j.molp.2014.11.023
摘要

In this study, we identified a defense-related major latex protein (MLP) from upland cotton (designated GhMLP28) and investigated its functional mechanism. GhMLP28 transcripts were ubiquitously present in cotton plants, with higher accumulation in the root. Expression of the GhMLP28 gene was induced by Verticillium dahliae inoculation and was responsive to defense signaling molecules, including ethylene, jasmonic acid, and salicylic acid. Knockdown of GhMLP28 expression by virus-induced gene silencing resulted in increased susceptibility of cotton plants to V. dahliae infection, while ectopic overexpression of GhMLP28 in tobacco improved the disease tolerance of the transgenic plants. Further analysis revealed that GhMLP28 interacted with cotton ethylene response factor 6 (GhERF6) and facilitated the binding of GhERF6 to GCC-box element. Transient expression assay demonstrated that GhMLP28 enhanced the transcription factor activity of GhERF6, which led to the augmented expression of some GCC-box genes. GhMLP28 proteins were located in both the nucleus and cytoplasm and their nuclear distribution was dependent on the presence of GhERF6. Collectively, these results demonstrate that GhMLP28 acts as a positive regulator of GhERF6, and synergetic actions of the two proteins may contribute substantially to protection against V. dahliae infection in cotton plants.
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