Synthesis and pharmacological evaluation of S-nitrosothiols for their smooth muscle relaxant activity on rat tracheal chain

The gaseous molecule nitric oxide (NO) is a highly reactive molecule that is synthesized by a variety of biological tissues and it modulates a large variety of physiol ogical functions including vascular tone, intestina l motility, platelet aggregation, proliferation, apoptosis, and neurotransmission. Its physiological effects are a ttributed to a very small, freely diffusible, and potentially high ly reactive radical. NO regulation, signal transduc tion, and cytotoxicity is strictly dependent upon its chemica l reactivity with oxygen and metals rather than spe cific structural instructions with physiological targets. The S-Nitr osothiols are the biological metabolites of nitric oxide. They represent a more stable metabolite of nitric oxide that can either be stored, or transported in the fo rm of Snitrosothiols (e.g. S-Nitrosocysteine in the body). Various hybrid copounds of S-Nitrosothiols such as SNitrosomercaptobenzothiazole and S-Nitrosomercaptob enzimidazole have been synthesized in the laborator y. The structure of the synthesised compounds was confirme d through the interpretation of spectral data. Prep ared compunds were then evaluated for the smooth muscle relaxant activity on rat tracheal chain. The paired t-test value for SNAP (S-Nitroso-Acetylpenicillamine) as standar d and SNMBT was found to be 0.878 from t-distributi on table, p = 0.3 for degree of freedom, F = 5 the value of t is between 0.559. The calculated value of t is gre ater than this which indicated that significant level is more than 98%.