聚唾液酸
神经细胞粘附分子
胶质瘤
转染
癌症研究
细胞培养
细胞粘附分子
生物
细胞
细胞粘附
病理
免疫学
医学
生物化学
遗传学
作者
Masami Suzuki,Misa Suzuki,Jun Nakayama,Atsushi Suzuki,Kiyohiko Angata,Shihao Chen,Keiichi Sakai,Kazuki Hagihara,Yu Yamaguchi,Minoru Fukuda
出处
期刊:Glycobiology
[Oxford University Press]
日期:2005-05-04
卷期号:15 (9): 887-894
被引量:140
标识
DOI:10.1093/glycob/cwi071
摘要
Polysialic acid (PSA) is thought to attenuate neural cell adhesion molecule (NCAM) adhesion, thereby facilitating neural cell migration and regeneration. Although the expression of PSA has been shown to correlate with the progression of certain tumors such as small cell lung carcinoma, there have been no studies to determine the roles of PSA in gliomas, the most common type of primary brain tumor in humans. In this study, we first revealed that among patients with glioma, PSA was detected more frequently in diffuse astrocytoma cells, which spread extensively. To determine directly the role of PSA in glioma cell invasion, we transfected C6 glioma cells with polysialyltransferases to express PSA. In those transfected cells, PSA is attached mainly to NCAM-140, whereas the mock-transfected C6 cells express equivalent amounts of PSA-free NCAM-140. Both PSA negative and positive C6 cell lines exhibited almost identical growth rates measured in vitro. However, PSA positive C6 cells exhibited increased invasion to the corpus callosum, where the mock-transfected C6 glioma cells rarely invaded when inoculated into the brain. By contrast, the invasion to the corpus callosum by both the mock-transfected and PSA positive C6 cells was observed in NCAM-deficient mice. These results combined indicate that PSA facilitates tumor invasion of glioma in the brain, and that NCAM–NCAM interaction is likely attenuated in the PSA-mediated tumor invasion.
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