Randomized controlled study for the anti‐aging effect of human adipocyte‐derived mesenchymal stem cell media combined with niacinamide after laser therapy

Abstract Background Recent advancements in laser‐assisted drug delivery have emphasized the importance of post‐laser use of active ingredients. Aims We evaluated the effect of topical post‐laser treatment with adipocyte‐derived stem cell‐containing medium (ADSC‐CM) in combination with niacinamide through a double‐blind, randomized, vehicle‐controlled study. Patients/Methods Twenty‐five patients with aging skin underwent ablative fractional laser (AFL) treatment on both sides of the face for skin rejuvenation. Moisturizers with or without ADSC‐CM plus 2% niacinamide was applied to the designated sides of faces for 3 weeks. The wrinkle and the melanin indices were assessed using a specialized digital photography analyzer. Additional in vitro assays were performed. Results The wrinkle index, melanin index, patient satisfaction score, and the investigator's global esthetic improvement scale (GAIS) after use of ADSC‐CM plus niacinamide were significantly higher than after use of the vehicle cream. The in vitro UVB irradiation assays with human keratinocytes showed decreased levels of pro‐inflammatory cytokines upon incubation with ADSC‐CM plus niacinamide, and the cell scratch assay displayed decreased MMP‐1 and MMP‐2 expression as well as increased Type 1 collagen expression. Conclusions We demonstrated that post‐laser topical application of ADSC‐CM in combination with niacinamide has anti‐aging effect on skin.