Effect and mechanism of rapamycin on proliferation and apoptosis of human lung cancer cells

细胞凋亡 分子生物学 膜联蛋白 信使核糖核酸 细胞生长 生物 肺癌 免疫组织化学 男科 癌症研究 基因 内科学 免疫学 医学 生物化学
作者
Wenning Cao,Lan Ma
出处
期刊:Cellular and Molecular Biology 卷期号:66 (6): 65-70 被引量:1
标识
DOI:10.14715/cmb/2020.66.6.12
摘要

The purpose of this study was to investigate the effects of RAPA on the proliferation and the expression of p53, Bcl-2 and Bax proteins in cultured human small cell lung cancer (NCI-H446) cells, and to explore the possible mechanism of RAPA-treated NCI-H446 cells with different concentrations of RAPA-treated NCI-H446 cells. The proliferation of NCI-H446 cells in all groups was assayed by the CCK-8 method. FITC-Annexin V/PI double staining method was used to determine the apoptosis of NCI-H446 cells. The immunohistochemical SP method was used to detect the expression of p53, Bcl-2 and Bax. Expression of p53, Bcl-2 and Bax mRNA was detected by RT-PCR. The results showed that, after 48h treatment, the proliferation of NCI-H446 cells treated with 5ng/mL, 10ng/mL and 15ng/mL RAPA decreased significantly (P < 0.05) and the proliferation inhibition rate increased significantly (P < 0.05) compared with the control group, and the proliferation inhibition rate had a dose-dependent relationship with RAPA. Compared with the control group, the apoptosis rate of NCI-H446 cells treated with 5ng/mL, 10ng/mL and 15ng/mL RAPA increased significantly (P < 0.05), and there was a dose-dependent relationship between the apoptosis rate and RAPA. The expression of Bcl-2 protein and mRNA was higher in the control group, while the expression of p53 and Bax protein and mRNA was lower. The expression of Bcl-2 protein and mRNA decreased and the expression of p53 and Bax protein and mRNA increased gradually with the increase of concentration and the prolongation of action time in 5ng/mL, 10ng/mL and 15ng/mL RAPA groups. In the control group, the intracellular Ca2+ concentration was constant, and there was no significant change with time; while in the 5ng / mL, 10ng / mL, and 15ng / mL RAPA group, the intracellular Ca2+ concentration in the RAPA group increased significantly after 12 h of administration (P <0.05); After that, with the prolonged action time of the medicine, the intracellular Ca2+ concentration in the 5ng / mL, 10ng / mL, and 15ng / mL RAPA group decreased, but at 72h, the effect was 5ng / mL, 10ng / mL, and 15ng / mL RAPA. The intracellular Ca2+ fluorescence intensity in the group was still significantly higher than that in the control group (P <0.05). In conclusion, RAPA can induce apoptosis of NCI-H446 cells by down-regulating Bcl-2 gene expression, up-regulating P53 and Bax gene expression, and increasing intracellular Ca2+ concentration and its apoptosis induction effect have timeliness and dose-effect.

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
卢雨生完成签到,获得积分10
3秒前
Twilight发布了新的文献求助10
4秒前
满意的惮完成签到 ,获得积分10
5秒前
5秒前
机智的友容完成签到 ,获得积分10
5秒前
6秒前
活力的以寒完成签到 ,获得积分10
6秒前
Harper完成签到,获得积分10
7秒前
7秒前
贪玩曼梅应助幸福安康采纳,获得10
9秒前
高无怨发布了新的文献求助30
12秒前
竹竹完成签到,获得积分10
12秒前
三和小神发布了新的文献求助10
12秒前
13秒前
李健的小迷弟应助wing采纳,获得10
17秒前
旦皋完成签到 ,获得积分10
17秒前
粥小周发布了新的文献求助10
17秒前
18秒前
18秒前
19秒前
LWS666发布了新的文献求助10
19秒前
hhh完成签到,获得积分20
21秒前
24秒前
啦啦啦完成签到 ,获得积分10
24秒前
科研小白完成签到,获得积分10
24秒前
一木完成签到,获得积分10
25秒前
25秒前
hanggg完成签到,获得积分10
25秒前
俊杰完成签到,获得积分20
25秒前
dandna完成签到 ,获得积分10
26秒前
迷路以筠完成签到,获得积分10
27秒前
粥小周完成签到,获得积分10
28秒前
hanggg发布了新的文献求助10
28秒前
哆啦A梦发布了新的文献求助20
29秒前
西瓜啵啵完成签到,获得积分10
29秒前
susu完成签到 ,获得积分10
30秒前
hanhuikoo发布了新的文献求助10
30秒前
俊杰发布了新的文献求助10
31秒前
lwl666完成签到,获得积分10
32秒前
32秒前
高分求助中
Continuum Thermodynamics and Material Modelling 3000
Production Logging: Theoretical and Interpretive Elements 2700
Les Mantodea de Guyane Insecta, Polyneoptera 1000
Structural Load Modelling and Combination for Performance and Safety Evaluation 1000
Conference Record, IAS Annual Meeting 1977 820
England and the Discovery of America, 1481-1620 600
電気学会論文誌D(産業応用部門誌), 141 巻, 11 号 510
热门求助领域 (近24小时)
化学 材料科学 生物 医学 工程类 有机化学 生物化学 物理 纳米技术 计算机科学 内科学 化学工程 复合材料 基因 遗传学 物理化学 催化作用 量子力学 光电子学 冶金
热门帖子
关注 科研通微信公众号,转发送积分 3572296
求助须知:如何正确求助?哪些是违规求助? 3142501
关于积分的说明 9448015
捐赠科研通 2843973
什么是DOI,文献DOI怎么找? 1563103
邀请新用户注册赠送积分活动 731630
科研通“疑难数据库(出版商)”最低求助积分说明 718640