谷胱甘肽
氧化应激
代谢途径
细胞内
药理学
代谢组学
毒性
抗氧化剂
化学
活力测定
新陈代谢
生物化学
细胞凋亡
生物
酶
有机化学
色谱法
作者
Hongrong Long,Zhongquan Xin,Fan Zhang,Zhenya Zhai,Xiaojun Ni,Jialuo Chen,Kang Yang,Pinfeng Liao,Limeng Zhang,Zaili Xiao,Daniel Sindaye,Baichuan Deng
出处
期刊:Food Chemistry
[Elsevier]
日期:2020-09-17
卷期号:338: 128116-128116
被引量:23
标识
DOI:10.1016/j.foodchem.2020.128116
摘要
In this study, we investigated the cytoprotective effects of dihydromyricetin (DHM) against deoxynivalenol (DON)-induced toxicity and accompanied metabolic pathway changes in porcine jejunum epithelial cells (IPEC-J2). The cells were incubated in 250 ng/ml DON cotreated with 40 µM DHM, followed by toxicity analysis, oxidative stress reaction analysis, inflammatory response analysis and metabolomic analysis. The results showed that DHM significantly increased the cell viability (P < 0.01), the intracellular GSH level (P < 0.01) and decreased the intracellular ROS level (P < 0.01), the secretion of TNF-α, IL-8 (P < 0.01) and the apoptotic cell percentages (P < 0.01) in IPEC-J2 cells compared to that in the DON group. Metabolomic analysis revealed that DHM recovered the disorder of metabolic pathways such as glutamate metabolism, arachidonic metabolism and histidine metabolism caused by DON. In summary, DHM alleviated cell injury induced by DON and it is possibly through its antioxidant activity, anti-inflammatory activity or ability to regulate metabolic pathways.
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