[Preparation of magnetic nanoparticles based on MIL-100(Fe) and its application in the enrichment of endogenous peptides].

Core-shell magnetic nanoparticles, Fe3O4@MIL-100(Fe), were synthesized by layer-by-layer self-assembly. The final material, Fe3O4@MC, was obtained after high temperature calcination. Fe3O4@MC magnetic nanoparticles exhibited large pore size (17.78 nm) and high carbon content (6.79%). Thirty-three peptides of bovine serum albumin (BSA) digest were enriched by Fe3O4@MC magnetic nanomaterial. Fe3O4@MC magnetic nanomaterial also showed high selectivity for peptides when the mass ratio of BSA digest to BSA was 1:400. In addition, the nanomaterial material exhibited excellent enrichment performance for the endogenous peptides of human serum.