Responses of bacterial communities and organic matter degradation in surface sediment to Macrobrachium nipponense bioturbation

The excessive accumulation of organic matter (OM) in sediments in aquaculture ponds is a potential environmental threat due to the risk of endogenous water pollution and eutrophication. From the perspective of inhibiting OM accumulation to prevent endogenous water pollution, the present study investigated the OM degradation states, variations of bacterial communities and basic environmental factors in sediments with/without Macrobrachium nipponense treatment/control groups in triplicate for effects of bioturbation on OM degradation in 90-day incubation. The total organic carbon (TOC) and total nitrogen (TN) in the M. nipponense treatment were higher than in the control at the 30th and 60th days, while no significant differences between treatment and control were found at the end of the experiment. Significantly higher oxidation-reduction potential (ORP) and more extensively degraded OM were observed in the M. nipponense treatment. Eleven significantly differential bacterial taxa were enriched in the sediments of M. nipponense treatment, of which eight (Actinobacteria, Chitinophagales, Chitinophagaceae, Flavihumibacter, Marinilabiliaceae, Cytophaga xylanolytica group, Christensenellaceae, and Christensenellaceae R-7 group) were significantly correlated with at least two OM degradation indicators. The functional groups chemoheterotrophy, aerobic chemoheterotrophy, xylanolysis, ureolysis, and intracellular parasites were enhanced by M. nipponense and were negatively correlated with OM degradation indictors. Overall, the M. nipponense bioturbation effectively increased the ORP to provide better conditions for OM degradation, altered the taxonomic composition and functional groups to enhance the bacterial ability for OM degradation, and finally promoted the OM degradation of the surface sediment in an artificial aquaculture system.