Measurement of Mouse and Human Interleukin 10

Abstract Interleukin 10 (IL‐10) is a multifunctional cytokine produced by T cells and other cell types. It regulates activities of a variety of the cells of the immune system and may play a crucial role in cross‐regulation of different types of T cells. Two methods of quantitating IL‐10 are provided in this unit. The first is an enzyme‐linked immunosorbent assay (ELISA) using a pair of monoclonal antibodies specific for mouse IL‐10. This method has the advantages of high specificity and insensitivity to other cytokines present in test samples. The second method is a bioassay which has the advantages that both human and mouse IL‐10 are detected and that functional protein is measured. Both methods have similar sensitivity.