吲哚青绿
肝细胞癌
光漂白
材料科学
荧光寿命成像显微镜
拉曼散射
分子成像
显像剂
荧光
拉曼光谱
肝肿瘤
肝癌
纳米颗粒
共焦
纳米技术
病理
生物医学工程
医学
癌症研究
体内
光学
生物
生物技术
物理
作者
Chrysafis Andreou,Volker Neuschmelting,Darjus F. Tschaharganeh,Chun‐Hao Huang,Anton Oseledchyk,Pasquale Iacono,Hazem Karabeber,Rivka R. Colen,Lorenzo Mannelli,Scott W. Lowe,Moritz F. Kircher
出处
期刊:ACS Nano
[American Chemical Society]
日期:2016-05-10
卷期号:10 (5): 5015-5026
被引量:149
标识
DOI:10.1021/acsnano.5b07200
摘要
Complete surgical resection is the ideal first-line treatment for most liver malignancies. This goal would be facilitated by an intraoperative imaging method that enables more precise visualization of tumor margins and detection of otherwise invisible microscopic lesions. To this end, we synthesized silica-encapsulated surface-enhanced Raman scattering (SERS) nanoparticles (NPs) that act as a molecular imaging agent for liver malignancies. We hypothesized that, after intravenous administration, SERS NPs would avidly home to healthy liver tissue but not to intrahepatic malignancies. We tested these SERS NPs in genetically engineered mouse models of hepatocellular carcinoma and histiocytic sarcoma. After intravenous injection, liver tumors in both models were readily identifiable with Raman imaging. In addition, Raman imaging using SERS NPs enabled detection of microscopic lesions in liver and spleen. We compared the performance of SERS NPs to fluorescence imaging using indocyanine green (ICG). We found that SERS NPs delineate tumors more accurately and are less susceptible to photobleaching. Given the known advantages of SERS imaging, namely, high sensitivity and specific spectroscopic detection, these findings hold promise for improved resection of liver cancer.
科研通智能强力驱动
Strongly Powered by AbleSci AI