A simple, step-by-step dissection protocol for the rapid isolation of mouse dorsal root ganglia

脊髓 医学 椎板切除术 神经科学 感觉系统 背根神经节 解剖 生物
作者
James N. Sleigh,Greg A. Weir,Giampietro Schiavo
出处
期刊:BMC Research Notes [Springer Nature]
卷期号:9 (1) 被引量:117
标识
DOI:10.1186/s13104-016-1915-8
摘要

The cell bodies of sensory neurons, which transmit information from the external environment to the spinal cord, can be found at all levels of the spinal column in paired structures called dorsal root ganglia (DRG). Rodent DRG neurons have long been studied in the laboratory to improve understanding of sensory nerve development and function, and have been instrumental in determining mechanisms underlying pain and neurodegeneration in disorders of the peripheral nervous system. Here, we describe a simple, step-by-step protocol for the swift isolation of mouse DRG, which can be enzymatically dissociated to produce fully differentiated primary neuronal cultures, or processed for downstream analyses, such as immunohistochemistry or RNA profiling. After dissecting out the spinal column, from the base of the skull to the level of the femurs, it can be cut down the mid-line and the spinal cord and meninges removed, before extracting the DRG and detaching unwanted axons. This protocol allows the easy and rapid isolation of DRG with minimal practice and dissection experience. The process is both faster and less technically challenging than extracting the ganglia from the in situ column after performing a dorsal laminectomy. This approach reduces the time required to collect DRG, thereby improving efficiency, permitting less opportunity for tissue deterioration, and, ultimately, increasing the chances of generating healthy primary DRG cultures or high quality, reproducible experiments using DRG tissue.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
文艺书雪发布了新的文献求助10
刚刚
少寒完成签到,获得积分10
1秒前
2秒前
顺心凡灵完成签到,获得积分10
3秒前
泡沫完成签到,获得积分10
4秒前
研友_nvkJrZ发布了新的文献求助10
5秒前
SciGPT应助三石采纳,获得10
5秒前
Clover04应助aaa采纳,获得10
6秒前
凸迩丝儿完成签到,获得积分10
6秒前
菜菜Cc发布了新的文献求助10
6秒前
suntee完成签到,获得积分10
6秒前
7秒前
7秒前
7秒前
7秒前
沉默的含巧完成签到,获得积分10
8秒前
8秒前
9秒前
田様应助优秀不愁采纳,获得10
9秒前
白潇潇发布了新的文献求助10
10秒前
10秒前
充电宝应助科研通管家采纳,获得10
11秒前
元谷雪应助科研通管家采纳,获得10
11秒前
Singularity应助科研通管家采纳,获得20
11秒前
科研通AI2S应助科研通管家采纳,获得10
11秒前
慕青应助科研通管家采纳,获得10
11秒前
Singularity应助科研通管家采纳,获得20
11秒前
汉堡包应助科研通管家采纳,获得10
11秒前
科研通AI2S应助科研通管家采纳,获得10
11秒前
11秒前
11秒前
11秒前
12秒前
昌升完成签到,获得积分20
12秒前
kmkz发布了新的文献求助10
12秒前
13秒前
13秒前
suntee发布了新的文献求助10
13秒前
13秒前
13秒前
高分求助中
Sustainability in Tides Chemistry 2800
The Young builders of New china : the visit of the delegation of the WFDY to the Chinese People's Republic 1000
юрские динозавры восточного забайкалья 800
English Wealden Fossils 700
Foreign Policy of the French Second Empire: A Bibliography 500
Chen Hansheng: China’s Last Romantic Revolutionary 500
XAFS for Everyone 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3143605
求助须知:如何正确求助?哪些是违规求助? 2795002
关于积分的说明 7813063
捐赠科研通 2451122
什么是DOI,文献DOI怎么找? 1304258
科研通“疑难数据库(出版商)”最低求助积分说明 627213
版权声明 601386