基督教牧师
病毒学
聚合酶链反应
套式聚合酶链反应
医学
俄罗斯联邦
实时聚合酶链反应
克里米亚-刚果出血热
生物
地理
生物化学
哲学
神学
区域科学
滴答声
基因
作者
Karan' Ls,German A. Shipulin,Alexander E. Platonov
出处
期刊:PubMed
日期:2003-10-01
卷期号: (10): 50-4
摘要
Our group developed, within the present case study, two techniques' variations, i.e. a single-step RT-PCR and nested RT-PCR assays, for the purpose of detecting the Crimean-Congo hemorrhagic fever RNA virus in human samples. The above assays as well as those previously recommended by the Ministry of Health of the Russian Federation were simultaneously used in 14 clinical samples obtained from patients with Crimean hemorrhagic fever. After assessing the detection accuracy, it was found that the developed-by-us test system displayed the same or even better diagnostic values versus the previously recommended nested RT-PCR and a 1000-fold advantage over the previously recommended single-step RT-PCA. The single-step RT-PCR assay variation is always more preferable in sense of technical and economic motivations. Finally, the test system developed by us has every reason to become the method of choice in routine PCR diagnosis of Crimean hemorrhagic fever after all official trials and approvals are duly complied with.
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