RNA干扰
生物
秀丽隐杆线虫
黑腹果蝇
转基因
基因
基因组
遗传筛选
遗传学
模式生物
计算生物学
核糖核酸
表型
作者
Georg Dietzl,Doris Chen,Frank Schnorrer,Kuan-Chung Su,Yulia Barinova,Michaela Fellner,Beate Gasser,Kaolin Kinsey,Silvia Oppel,Susanne Scheiblauer,Africa Couto,Vincent Marra,Krystyna Keleman,Barry J. Dickson
出处
期刊:Nature
[Springer Nature]
日期:2007-07-01
卷期号:448 (7150): 151-156
被引量:2615
摘要
Forward genetic screens in model organisms have provided important insights into numerous aspects of development, physiology and pathology. With the availability of complete genome sequences and the introduction of RNA-mediated gene interference (RNAi), systematic reverse genetic screens are now also possible. Until now, such genome-wide RNAi screens have mostly been restricted to cultured cells and ubiquitous gene inactivation in Caenorhabditis elegans. This powerful approach has not yet been applied in a tissue-specific manner. Here we report the generation and validation of a genome-wide library of Drosophila melanogaster RNAi transgenes, enabling the conditional inactivation of gene function in specific tissues of the intact organism. Our RNAi transgenes consist of short gene fragments cloned as inverted repeats and expressed using the binary GAL4/UAS system. We generated 22,270 transgenic lines, covering 88% of the predicted protein-coding genes in the Drosophila genome. Molecular and phenotypic assays indicate that the majority of these transgenes are functional. Our transgenic RNAi library thus opens up the prospect of systematically analysing gene functions in any tissue and at any stage of the Drosophila lifespan.
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