细胞粘附
粘附
植物细胞
生物物理学
脚手架
细胞
细胞壁
材料科学
化学
细胞生物学
生物医学工程
生物
生物化学
医学
基因
复合材料
作者
Ryan Calcutt,Richard Vincent,Derrick Dean,Treena Livingston Arinzeh,Ram Dixit
出处
期刊:Science Advances
[American Association for the Advancement of Science (AAAS)]
日期:2021-10-22
卷期号:7 (43)
被引量:19
标识
DOI:10.1126/sciadv.abj1469
摘要
Mechanistic studies of plant development would benefit from an in vitro model that mimics the endogenous physical interactions between cells and their microenvironment. Here, we present artificial scaffolds to which both solid- and liquid-cultured tobacco BY-2 cells adhere without perturbing cell morphology, division, and cortical microtubule organization. Scaffolds consisting of polyvinylidene tri-fluoroethylene (PVDF-TrFE) were prepared to mimic the cell wall’s fibrillar structure and its relative hydrophobicity and piezoelectric property. We found that cells adhered best to scaffolds consisting of nanosized aligned fibers. In addition, poling of PVDF-TrFE, which orients the fiber dipoles and renders the scaffold more piezoelectric, increased cell adhesion. Enzymatic treatments revealed that the plant cell wall polysaccharide, pectin, is largely responsible for cell adhesion to scaffolds, analogous to pectin-mediated cell adhesion in plant tissues. Together, this work establishes the first plant biomimetic scaffolds that will enable studies of how cell-cell and cell-matrix interactions affect plant developmental pathways.
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