Reduction of the resistome risk from cow slurry and manure microbiomes to soil and vegetable microbiomes

抵抗性 肥料 生物 微生物群 土壤微生物学 生物技术 泥浆 基因组 兽医学 农学 抗生素耐药性 抗生素 土壤水分 微生物学 生态学 环境科学 环境工程 整合子 生物信息学 遗传学 基因 医学
作者
Leire Jauregi,Lur Epelde,Aitor González,José Luis Lavín,Carlos Garbisu
出处
期刊:Environmental Microbiology [Wiley]
卷期号:23 (12): 7643-7660 被引量:6
标识
DOI:10.1111/1462-2920.15842
摘要

In cow farms, the interaction between animal and environmental microbiomes creates hotspots for antibiotic resistance dissemination. A shotgun metagenomic approach was used to survey the resistome risk in five dairy cow farms. To this purpose, 10 environmental compartments were sampled: 3 of them linked to productive cows (fresh slurry, stored slurry, slurry-amended pasture soil); 6 of them to non-productive heifers and dry cows (faeces, fresh manure, aged manure, aged manure-amended orchard soil, vegetables-lettuces and grazed soil); and, finally, unamended control soil. The resistome risk was assessed using MetaCompare, a computational pipeline which scores the resistome risk according to possible links between antibiotic resistance genes (ARGs), mobile genetic elements (MGEs) and human pathogens. The resistome risk decreased from slurry and manure microbiomes to soil and vegetable microbiomes. In total (sum of all the compartments), 18,157 ARGs were detected: 24% related to ansamycins, 21% to multidrugs, 14% to aminoglycosides, 12% to tetracyclines, 9% to β-lactams, and 9% to macrolide-lincosamide-streptogramin B. All but two of the MGE-associated ARGs were only found in the animal dejections (not in soil or vegetable samples). Several ARGs with potential as resistome risk markers (based on their presence in hubs of co-occurrence networks and high dissemination potential) were identified. As a precautionary principle, improved management of livestock dejections is necessary to minimize the risk of antibiotic resistance.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
yuuu发布了新的文献求助10
刚刚
CCC完成签到,获得积分20
2秒前
8R60d8应助布布爱吃炸鸡采纳,获得10
3秒前
灯火入眉弯完成签到,获得积分10
4秒前
12秒前
狗窝里的猫yan应助Hyll采纳,获得10
15秒前
STAN完成签到,获得积分10
17秒前
小二郎应助傲慢大铁锤采纳,获得30
18秒前
REcup完成签到,获得积分10
18秒前
朱博发布了新的文献求助10
19秒前
要减肥半仙完成签到,获得积分10
20秒前
21秒前
动听如之完成签到 ,获得积分10
24秒前
24秒前
Lucas应助ZHI采纳,获得10
24秒前
科目三应助juan采纳,获得10
29秒前
29秒前
29秒前
31秒前
xiaohao完成签到 ,获得积分10
31秒前
桐桐应助KD采纳,获得10
33秒前
初余发布了新的文献求助10
36秒前
小黄发布了新的文献求助10
37秒前
小蘑菇应助闫111采纳,获得10
40秒前
小燕子完成签到 ,获得积分10
42秒前
44秒前
47秒前
48秒前
48秒前
ZHI完成签到,获得积分20
49秒前
50秒前
50秒前
KD发布了新的文献求助10
53秒前
WRZ发布了新的文献求助10
54秒前
小马完成签到,获得积分10
54秒前
55秒前
NexusExplorer应助北执采纳,获得10
56秒前
John完成签到,获得积分10
56秒前
57秒前
共享精神应助盆浴烟采纳,获得10
58秒前
高分求助中
Sustainability in Tides Chemistry 1500
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
CLSI EP47 Evaluation of Reagent Carryover Effects on Test Results, 1st Edition 800
Threaded Harmony: A Sustainable Approach to Fashion 799
Livre et militantisme : La Cité éditeur 1958-1967 500
Retention of title in secured transactions law from a creditor's perspective: A comparative analysis of selected (non-)functional approaches 500
"Sixth plenary session of the Eighth Central Committee of the Communist Party of China" 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3055401
求助须知:如何正确求助?哪些是违规求助? 2712227
关于积分的说明 7430195
捐赠科研通 2357037
什么是DOI,文献DOI怎么找? 1248528
科研通“疑难数据库(出版商)”最低求助积分说明 606737
版权声明 596093