清脆的
核糖核酸
核酸
DNA
劈开
化学
劈理(地质)
分子生物学
回文
抄写(语言学)
计算生物学
Cas9
生物
引导RNA
核酸酶
细胞生物学
反式激活crRNA
寡核苷酸
生物物理学
裂解和多聚腺苷酸化特异性因子
CRISPR干扰
基因
生物化学
哲学
古生物学
断裂(地质)
语言学
作者
Yangdao Wei,Zhiqing Yang,Chengli Zong,Buhua Wang,Xiaolin Ge,Xiao Tan,Xin Liu,Zhenzhen Tao,Peng Wang,Chunxin Ma,Yi Wan,Jinghong Li
标识
DOI:10.1002/anie.202110384
摘要
As a CRISPR-Cas system (clustered regularly interspaced short palindromic repeats and CRISPR associated proteins), Cas14a1 can cis/trans cleave single-stranded DNA (ssDNA). Here, we describe an unreported capacity of Cas14a1: RNA can trigger the trans ssDNA cleavage. This Cas14a1-based RNA-activated detection platform (Amplification, Transcription, Cas14a1-based RNA-activated trans ssDNA cleavage, ATCas-RNA) has an outstanding specificity for the detection of target RNAs with point mutation resolution, which is better than that of the Cas14a1-based ssDNA-activation. Using ATCas-RNA via a fluorophore quencher-labeled ssDNA reporter (FQ), we were able to detect 1 aM pathogenic nucleic acid within 1 h, and achieve 100 % accuracy with 25 milk samples. This platform can serve as a new tool for high-efficiency nucleic acid diagnostics. Importantly, this work can expand our understanding of Cas14a1 and inspire further mechanisms and applications of Class-2 Cas systems.
科研通智能强力驱动
Strongly Powered by AbleSci AI