桥甾醇
甾醇
羊毛甾醇
酶
生物化学
还原酶
胆固醇
化学
生物发生
基质(水族馆)
底物特异性
生物
生态学
基因
作者
Daniel Steinberg,Joel Avigan
标识
DOI:10.1016/s0076-6879(69)15023-5
摘要
The biological reduction of desmosterol is readily demonstrated in the rat by injecting the labeled sterol intraportally and isolating radioactive cholesterol from the liver. The 24,25-double bond in a variety of sterols can be reduced by the same enzyme, and the more general name “sterol Δ -24-reductase” is more appropriate. Until the enzyme is solubilized and purified, it should be recognized that the name refers only to activities demonstrable in particulate preparations. As the substrates are added in suspension, little can be said with respect to kinetics or intimate mechanism of action of the enzyme. This chapter discusses its properties, substrate specificity, susceptibility to specific inhibitors, and role in sterol biogenesis. The chapter describes assay based on that employed by Steinberg, Avigan, and Goodman for reduction of desmosterol or lanosterol.
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